The Universal
UDP-Glycosyltransferase Assay is a single step, homogenous,
fluorescent assay for detection and screening of UDP-producing
enzymes. Direct detection of UDP with a fluorescence polarization
(FP) readout provides a safe, HTS-compatible alternative to
cumbersome radioassay methods and is more sensitive and less
subject to interference than coupled assay methods, where the UDP
is converted to another product. Based on Creative BioMart
immunodetection technology, the Universal UDP-Glycosyltransferase
Assay is compatible with any enzyme class that produces UDP,
including UDP-glucose-, UDP-galactose- and
UDP-glucuronosyl-transferases as well as glycogen, hyaluranon and
cellulose synthases.