#90 new
creativebiostructure

DEPT and APT NMR Service

Reported by creativebiostructure | January 25th, 2024 @ 06:12 AM

Distortionless enhancement of polarization transfer (DEPT) is a detection technique in carbon spectroscopy NMR spectroscopy. It can be used to determine the diversity of carbon atoms, i.e., whether they are primary (methyl or -CH3 group), secondary (methylene or -CH2 group), or tertiary (hypo methyl or -CH group). Three variants, DEPT-45, DEPT-90, and DEPT-135 NMR, are available.

DEPT NMR can be applied to identify compounds, especially for the designation and identification of some carbon signals with very complex structures containing different numbers of hydrogen atoms with simplicity and accuracy. DEPT NMR, although it cannot directly observe quaternary carbons (i.e., carbons without hydrogen), can accurately identify quaternary carbons in combination with the full map, and the DEPT map is clearer. It can overcome some difficulties in the overlapping of resonance lines in the partial resonance test and the fingerprinting of complex molecules. The measurement time is shorter than that of the partial resonance spectrum.

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